Project name: Homo sapiens

Description: Malignant cells show major disruptions in DNA methylation profiles which manifest as aberrant hypermethylation and hypomethylation of gene promoters as well as global genomic hypomethylation. To date, many genes with aberrant promoter hypermethylation have been identified in essentially all forms of cancer including cell cycle regulators, DNA repair genes, genes associated with apoptosis, hormonal regulation, detoxification, metastasis, angiogenesis, and many othersWe developed an effective high-resolution approach for mapping genome-wide, and cancer-specific DNA methylation profiles. We have used this approach to provide first genomic DNA methylation profiling in human paediatric osteosarcoma tumoursOverall design: We utilized this platform in combination with the methylated DNA immunoprecipitation (Me-DIP) to develop a comprehensive approach for detection of hypo- and hypermethylation changes at high resolution, and used it to detect such changes in human osteosarcoma tumours in relation to the normal human osteoblasts.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Medical

Release date: 2009-09-08

Last updated: 2008-09-19