Reconstruction of gene interaction networks by in vitro RNA interference and global expression analysis
Source: NCBI BioProject (ID PRJNA113581)
Source: NCBI BioProject (ID PRJNA113581)
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Project name: Homo sapiens
Description: We here used the technology of RNA interference or selective treatment of the ESR1 and cDNA microarrays to reconstruct a network between genes known to be disregulated in ER-positive breast cancer diseases. Therefor single genes of high importance were silenced and the effect of this pertubation was measured with cDNA microarrays.Keywords: Network Reconstruction, RNAi, siRNA, Interaction NetworkOverall design: human ER-positive breast cancer cell line was cultivated as described and transfection with chemically synthesized siRNAs was performed according to standard HiPerFect transfection protocol by Qiagen (Hilden, Germany) At least 4 cDNA-microarrays were hybridized in dye-swap method for the silencing of every gene of interest. To minimize off-target effects we used at least two different siRNAs per gene.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Medical
Organization: Molecular Genome Analysis, DKFZ
Release date: 2009-07-29
Last updated: 2008-07-30