Project name: Oryctolagus cuniculus

Description: The mechanisms through which cardioplegia affords cardioprotection is complex and can not be investigated as a single entity but rather must be investigated as a system. In order to improve understanding of the molecular processes modulating the effects of global ischemia and the cardioprotection afforded by cardioplegia we have constructed rabbit heart cDNA libraries and have isolated, sequenced and identified a compendium of non-redundant cDNAs for use in microarray analysis. Methods: New Zealand White rabbits were used for Langendorff perfusion consisting of 30 minutes global ischemia (GI)and 120 minutes reperfusion. Cardioplegia hearts received cardioplegia prior to GI. Control hearts were perfused without GI. The effects of RNA and protein synthesis on the cardioprotection afforded by cardioplegia were investigated separately by pre-perfusion with either alpha-amanitin or cycloheximide. Rabbit heart cDNA libraries were constructed and cDNAs isolated and identified by 5’ sequencing to create a compendium of non-redundant cDNAs for microarray analysis coupled with proteomic analysis. Results and Conclusion: Our studies demonstrate that the cardioprotection afforded by cardioplegia is modulated by RNA and protein dependent mechanisms. Microarray and proteomic enrichment analysis indicated that cardioplegia significantly increases differentially expressed genes/proteins associated with the mitochondrion and mitochondrial function and significantly up-regulates the biological processes of muscle contraction, involuntary muscle contraction, carboxylic acid and fatty acid catabolic processes, fatty acid beta-oxidation and fatty acid metabolic processes.Overall design: Rabbit heart (atria and ventricles):control vs. global ischemia; control vs. cardioplegia; cardioplegia vs. global ischemiaGSM364554 Rabbit slide 56 550_600_33 Raw data for Control vs. Global Ischemia (GI). Control+ Cy3; GI + Cy5.GSM364554 Rabbit slide 56 530_650_33 Raw data for Control vs. Global Ischemia (GI)-- Control= Cy3; GI=Cy5GSM364555 Rabbit slide 58 550_600_33 Raw data for Control vs. KMg+DZX (cardioplegia) Control=Cy3; KMg+DZX (cardioplegia)=Cy5.GSM364554 Rabbit slide 60 550_600_33 Raw data for Control vs GI Control =Cy3; GI=Cy5.GSM364555 Rabbit slide 62 520_680_33 Raw data for Control vs. KMg+DZX (Cardioplegia). Control Cy5; KMg+DZX (Cardioplegia)= Cy3.GSM364556 Rabbit slide 70 505_590_33 Raw data for Global Ischemia (GI) vs. KMg+DZX (Cardioplegia). GI +Cy3; KMg+DZX (Cardioplegia)= Cy5GSM364556 Rabbit slide 71 505_590_33 Raw data for Global Ischemia (GI) vs. KMg+DZX (Cardioplegia). GI +Cy5; KMg+DZX (Cardioplegia)= Cy3

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2009-01-28

Last updated: 2009-01-26