Project name: Saccharomyces cerevisiae

Description: DNA-DNA interactions are important for the control of gene expression. However, DNA interactions that occur in trans are difficult to identify. We used 4Cs to identify DNA regions that interact with the yeast ribosomal DNA enhancer.We used 4Cs to identify DNA regions that physically interact with the yeast ribosomal enhancer.Keywords: 4COverall design: Yeast cells were grown to exponential stage and cross linked, digested with Msp1, diluted and ligated. Fragments that interact with the ribosomal enhancer were amplified by nested PCR> Cut samples were used as a negative control. One set of samples were treated with phenanthroline prior to cross-linking.8 samples, 4 sets of 2 biological replicatesBackground controls: J0001.CEL, J0003.CEL, J0005.CEL, J0007.CELCut-ligated: J0002.CEL, J0004.CEL, J0006.CEL, J0008.CEL

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2009-04-08

Last updated: 2008-05-05