Project name: Streptomyces coelicolor

Description: The promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660. This vector was integrated into the chromosome of K24-1, a clean host version of K41-135 (the overproducer strain obtained from Kosan Biosciences). This strain was then grown in shake flask culture in R5 medium. RNA was harvested up through three days. The 12 h RNA sample was used as the reference sample for all RNA hybridizations. A genomic DNA vs. 12 h control was also done to determine initial relative transcript expression.Groups of assays that are related as part of a time series.Keywords: time_series_designOverall design: Computed

Data type: Other

Sample scope: Multiisolate

Relevance: Environmental

Release date: 2005-07-26

Last updated: 2005-07-25