Project name: Streptomyces coelicolor

Description: Saccharopolyspora erythraea NRRL2338 (wildtype) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) were grown in shake flask cultures in rich medium R5. RNA samples were harvested for each strain over 5 days. 12 h samples taken for each strain were used as their respective reference ("time zero") samples. The genomic DNA control compares NRRL2338 gDNA to NRRL2338 12 h RNA to show relative gene expression at the start of the timecourse. The 12 h RNA control compares initial gene expression between the wildtype and overproducer strains.Groups of assays that are related as part of a time series.Keywords: time_series_designOverall design: Computed

Data type: Other

Sample scope: Multiisolate

Relevance: Environmental

Release date: 2005-07-26

Last updated: 2005-07-25