Project name: Saccharomyces cerevisiae

Description: Arsenic is ubiquitously present in nature and various mechanisms haveevolved enabling cells to evade toxicity and acquire tolerance. Herein, weexplored how Saccharomyces cerevisiae (budding yeast) respond to trivalentarsenic (arsenite) by quantitative and kinetic transcriptome, proteome andsulfur metabolite profiling. Arsenite exposure affected transcription ofgenes encoding functions related to protein biosynthesis, arsenicdetoxification, oxidative stress defense, redox maintenance andproteolytic activity. Importantly, enzymes involved in sulfate assimilationand glutathione biosynthesis were induced at both gene and protein levels.Kinetic metabolic profiling evidenced a significant increase in the pools ofsulfur metabolites as well as elevated glutathione levels. Moreover, theflux in the sulfur assimilation pathway as well as the glutathione synthesisrate strongly increased with a concomitant reduction of sulfur incorporationinto proteins. By combining comparative genomics and molecular analyses, wepin-pointed transcription factors that mediate thecore of thetranscriptional response to arsenite. Taken together, our data reveals thatarsenite-exposed cells channel a large part of assimilated sulfur intoglutathione biosynthesis and we provide evidence that the transcriptionalregulators Yap1p and Met4p control this response in concert.Keywords: stress, time courseOverall design: Four time points, each array hybridized to untreated wild-type.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2007-10-18

Last updated: 2006-10-18