Project name: Homo sapiens

Description: Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity.Keywords: time courseOverall design: G-CSF mobilized peripheral blood CD34-positive cells were cultured with IL-3, IL-6, SCF and G-CSF under 20% O2. Three biological replicate experiments were analyzed and approximately 15% of the samples were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Release date: 2007-06-06

Last updated: 2006-09-26