Project name: Caenorhabditis elegans

Description: Background: A collection of genetic deficiencies covering over 70% of the Caenorhabditis elegans genome exists, however the application of these valuable biological tools has been limited due to the incomplete correlation between their genetic and physical characterization. !Series_summary = Results: We have applied oligonucleotide array Comparative Genomic Hybridization (oaCGH) to the high resolution, molecular characterization of several genetic deficiency and duplication strains in a 5Mb region of Chromosome III. We incorporate this data into a physical deficiency map which is subsequently used to direct the positional cloning of essential genes within the region. From this analysis we are able to quickly determine the molecular identity of several previously unidentified mutations.Conclusion: We have applied accurate, high resolution molecular analysis to the characterization of genetic mapping tools in Caenorhabditis elegans. Consequently we have generated a valuable physical mapping resource, which we have demonstrated can aid in the rapid molecular identification of mutations of interest.Keywords: C.elegans Deficiencies CGHOverall design: 7 test samples from heterozygous deficiency containing strains individually hybridized to reference DNA from strain JK2689 (5 hT2 balanced strains) or BC2842 (2 eT1 balanced strains)

Data type: Variation

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2007-10-11

Last updated: 2007-10-02