B cells sorted from myocardium of two LVAD recipients and the peripheral blood of these same recipients
Source: NCBI BioProject (ID PRJEB54024)
Source: NCBI BioProject (ID PRJEB54024)
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Project name: Cardiac and peripheral blood B cells from LVAD recipients
Description: The LVAD core collected at the time of LVAD implant was digested, PBMCs were isolated form peripheral blood of the same patient and "digested" similarly to the LVAD core. Cells were frozen in DMSO and media. Samples from two patients were collected. Eventually, cells were thawed, CD45+ CD19+ cells were FACS sorted and B cells from heart and blood were analyzed in the same 10X single cell sequencing reaction. Cells from heart and blood were marked with 2 different hashtag antibodies.
Data type: Other
Sample scope: Monoisolate
Organization: johns hopkins school of medicine
Last updated: 2022-08-09