Project name: Human memory B cells and circulating plasmablasts

Description: Memory B cells persist for a lifetime and rapidly differentiate into antibody-producing plasmablasts and plasma cells upon anti-gen re-encounter. The clonal relationship and evolution of memory B cells and circulating plasmablasts is not well understood.Using single-cell sequencing combined with isolation of specific antibodies, we found that in two healthy donors, the memoryB cell repertoire was dominated by large IgM, IgA and IgG2 clonal families, whereas IgG1 families, including those specific forrecall antigens, were of small size. Analysis of multiyear samples demonstrated stability of memory B cell clonal families andrevealed that a large fraction of recently generated plasmablasts was derived from long-term memory B cell families and wasfound recurrently. Collectively, this study provides a systematic description of the structure, stability and dynamics of thehuman memory B cell pool and suggests that memory B cells may be active at any time point in the generation of plasmablasts.

Data type: Other

Sample scope: Monoisolate

Last updated: 2022-06-08