RNA sequencing of inguinal white adipose tissue of diet-induced obese mouse . Effects of SF1 neuron ablation
Source: NCBI BioProject (ID PRJDB14088)
Source: NCBI BioProject (ID PRJDB14088)
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Project name: Mus musculus strain:STOCK Tg (Nr5a1-cre)7Lowl/J
Description: To investigate the role of steroidogenic factor 1 (SF1) - expressing neurons in the ventromedial hypothalamus (VMH) in inflammatory responses of adipose tissue in high-fat diet (HFD) -fed mice, SF1 neurons in the VMH were ablated by the injection of an adeno-associated virus (AAV) that expresses diphtheria toxin A (DTA) in the presence of Cre recombinase into the VMH of SF1-Cre mice (mice that express Cre under the control of the SF1 gene promoter). SF1 neuron-ablated and WT control mice were maintained on HFD for 10 weeks after allowing them to recover from virus injection for 5 weeks on a normal diet (ND) before tissue collection. Changes in gene expression in inguinal white adipose tissue (ingWAT) were then examined by RNA-sequencing analysis.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Medical
Organization: Division of Endocrinology and Metabolism, Department of Homeostatic Regulation, National Institute for Physiological Sciences
Release date: 2022-07-27
Last updated: 2022-07-26