Description: In this study, we performed a time series single-cell transcriptome experiment on Arabidopsis root explants that were induced on CIM (callus induction medium) for 0 day (CIM0), 1 day (CIM1), and 4 days (CIM4), and constructed a detailed single-cell transcriptional map of callus induction process. Based on this atlas, we successfully identified a cell type that responsible for the initiation of early callus: lateral root primordia-initiating like (LRPI-like) cells, and inferred their developmental trajectory. Further analysis found that LRPI-like cells is composed of cell subtypes at multiple developmental states which exhibited distinct pluripotency features, which revealed a cell lineage map of callus initiation. In addition, the transcription factor regulatory network analysis for LRPI-like cells revealed a number of transcription factors and their target genes involved in regulating the development of LRPI-like cells, such as LRP1. Collectively, the construction of cell lineage map of callus initiation gives unique glimpse of cell fate transition during callus formation, and these will further facilitate the identification of crucial regulators that promote callus formation.

Data type: Raw sequence reads; Transcriptome or Gene expression

Sample scope: Multiisolate

Release date: 2023-05-25

Last updated: 2023-05-25

Data size: 759.83GB