Project name: ALE-seq of moso bamboo plant material under stress conditions and during development

Description: Five-week-old moso bamboo (Phyllostachys edulis) seedlings from a single inbred plant were treated with heat stress at 42 degree centigrade for six hours, cold stress at 4 degree centigrade for sixteen hours, UV radiation under UV lamp (100 microwatt/cm2 and 50 cm distance to plant) for two hours, and salt stress by irrigating with 200 mM NaCl for three days. The seedlings grown at 25 degree centigrade with water irrigation were used as the control. The first three leaves from the top were collected. Calli generated from immature embryo of moso bamboo seeds were cultured in a medium containing 500 mg/L proline, 500 mg/L glutamine, 300 mg/L casein hydrolysate, 2 mg/L 2, 4-D, 0.1 mg/L zeatin, 30 g/L sucrose, and 8.0 g/L agar. Shoots of rapid growth stages were sampled at the 15th, 24th and 41st internodes, corresponding to the phases of initiation of cell division, rapid cell division and rapid cell elongation, respectively. The intercalary meristems in node slots at the early coloring stages (24th internode) were sampled from two internode color variation types, P. edulis f. luteosulcata (internode with yellow stripes) and P. edulis f. viridisulcata (internode with green stripes), and the wild type of moso bamboo. The intercalary meristems in shrunk and stretched parts of 15th twisted nodes, respectively, were sampled from an internode form mutation type (P. edulis cv. heterocycla) of moso bamboo with tortoise-shell-like internodes. In the meantime, the corresponding tissues of wild type moso bamboo were collected as the control group. All treatments and tissues were sampled with three biological replicates.

Data type: Raw sequence reads

Sample scope: Monoisolate

Relevance: Evolution

Release date: 2023-03-24

Last updated: 2023-03-24

Data size: 36.7GB