Description: Human pluripotent stem cell-derived islets (hPSC-islets) are a promising alternative to primary human islets for the treatment of insulin-deficient diabetes1-3. We previously demonstrated the feasibility of this approach in nonhuman primates. However, the therapeutic effects of hPSC-islets can be limited by the maladaptive processes at the transplantation site. Here, we demonstrate successfully implantation of hPSC-derived islets in a new transplantation site in the abdomen, the sub-anterior rectus sheath, in eight nonhuman primates. In this proof-of-principle study, we find that hPSC-islets survive and gradually mature after transplantation, leading to improved glycemic control in diabetic primates. The in situ gene expression analysis of hPSC-islet grafts under anterior rectus sheath in Monkey 5 and 6 using Stereo-seq. In human grafts, we observed the expression and enrichment of islet hormones, canonical islet-enriched transcription factors and genes involved in insulin processing, insulin secretion, glucose metabolism and oxidative phosphorylation, and ion channel.

Data type: Raw sequence reads

Sample scope: Multiisolate

Relevance: Medical

Release date: 2022-12-14

Last updated: 2022-11-02

Data size: 845.52GB