Project name: 266 cultured genomes and 482 metagenome-assembled genomes

Description: We performed a study of bacteria cultivation and genome sequencing along with deeply metagenome sequencing of 14 fecal samples from weanling piglet. 266 cultured genomes and 482 metagenome-assembled genomes (MAGs) were collected and clustered to 428 species across 10 phylum in this work. Genomic DNA of the 226 represent strains were extracted. The draft genomes were sequenced for 100 bp paired-end on DNBSEQ-T7 platform. The raw reads were filtered using SOAPnuke. After preliminary judgment of the genome size based on kmer, SPAdes was used to assembly. Metagenomic DNA of 14 samples were extracted and sequenced on DNBSEQ-T7 platform (CNP0002075). IDBA-UD were used to assembly and merge the optimal contig. MAGs were generated using three different tools configured in the 'binning' module of metaWRAP.

Data type: Genome sequencing and assembly; Genome sequencing; Assembly

Sample scope: Multiisolate

Relevance: Agricultural

Release date: 2021-12-03

Last updated: 2021-08-03

Data size: 503.76MB