Beta-lactamases: determination of their isoelectric points.
Antimicrob Agents Chemother, 1978/4;13(4):695-8.
Barthelemy M, Guionie M, Labia R
PMID: 96731
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Abstract
Important discrepancies in isoelectric points (pI) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like beta-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in PA-IEF. This has been clearly shown with beta-lactamase preparations from the following bacterial strains: Pseudomonas aeruginosa NCTC 8203 (pI = 8.7), P. aeruginosa NCTC 10701 (pI = 9.4), and Proteus morganii NCTC 235 (pI = 8.3). The data previously obtained by PA-IEF were much lower.
MeSH terms
Amidohydrolases; Cephalosporinase; Escherichia coli; Isoelectric Focusing; Isoelectric Point; Molecular Weight; Pseudomonas aeruginosa
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