[Immunologic study of the cellular components of bacillus pyocyaneus. III. Immunochemical analysis, toxicity and protective properties of water-soluble antigenic complexes].
Zh Mikrobiol Epidemiol Immunobiol, 1978/3;(3):65-70.
Stanislavskiĭ ES, Kolker II, Grishina IA, Zhvanetskaia MI
PMID: 96637
Abstract
Aqueous extracts of two Ps. aeruginosa strains killed with acetone were subjected to fractionation by preparative ultracentrifugation and gel-chromatography. Toxic activity of the extract was found to be connected with the high-molecular, possibly glycoprotein components reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 30--40% of rats against the generalized infection with Pseudomonas aeruginosa. This protective activity is apparently connected with the protein components (molecular weight--20000--60000 dalton), nontoxic for mice, not reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 60 to 80% of rats against Ps. aeruginosa infecsion. Thus, as a result of preparative ultracentrifugation and gel-chromatography it was postible to divide the toxic and the nontoxic protective components of Ps. aeruginosa.
MeSH terms
Animals; Antigens, Bacterial; Bacterial Proteins; Bacterial Toxins; Cell Fractionation; Chemical Phenomena; Chemistry; Chromatography, Gel; Mice; Molecular Weight; Precipitins; Pseudomonas aeruginosa; Rats; Ultracentrifugation
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