The use of an oscillating-tube densitometer as a tool in enzyme kinetics. Determination of the influence of sodium ascorbate on invertase, dextransucrase and dextranase.
J Biochem Biophys Methods, 1979/12;1(6):327-34.
PMID: 95345
Abstract
The use of a commercial oscillating-tube densitometer with an accuracy of 4 . 10(-7) g/cm3 for the determination of enzyme-kinetics constants is tested. This method is applied to the investigation of the influence of vitamin C (sodium ascorbate) on the glycolytic enzymes invertase, dextransucrase and dextranase. Invertase is inhibited uncompetitively, dextransucrase non-competitively. There is no significant effect of the vitamin on dextranase. The comparison of the mechanisms of the three enzymes suggests that only those reaction steps are inhibited by vitamin C in which fructose is released from the enzyme.
MeSH terms
Ascorbic Acid; Densitometry; Dextranase; Dextrans; Glucosyltransferases; Kinetics; Leuconostoc; Mathematics; Oscillometry; Penicillium; Sucrase; Sucrose
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