Transformation of the mycotoxin ochratoxin A in plants: 1. Isolation and identification of metabolites formed in cell suspension cultures of wheat and maize.
Nat Toxins, 1996;4(6):254-60.
Ruhland M[1], Engelhardt G, Schäfer W, Wallnöfer PR
Affiliations
PMID: 9029550
Abstract
The metabolism of the mycotoxin ochratoxin A in plant cells was investigated using cell suspension cultures of wheat and maize. A number of metabolites were detected by HPLC-chromatography with fluorescence detection. The main metabolites were ochratoxin alpha, ochratoxin A methyl ester, two isomers of hydroxyochratoxin A, and the glucosides and methyl esters of both hydroxyochratoxin A isomers. The compounds were isolated by TLC and preparative HPLC and identified by mass spectrometry and specific enzymic reactions.
MeSH terms
Biotransformation; Carbon Radioisotopes; Cells, Cultured; Chromatography, High Pressure Liquid; Mycotoxins; Ochratoxins; Radiometry; Spectrometry, Fluorescence; Triticum; Zea mays
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