Papain solubilization of the Epstein-Barr virus-induced membrane antigen.
J Virol, 1978/10;28(1):344-51.
PMID: 81320
Impact factor: 6.549
Abstract
The Epstein-Barr virus (EBV)-induced membrane antigen (MA) was successfully solubilized from the membranes of viable EBV-infected Raji cells by treatment with papain (5 to 6 U per 1 X 10(7) to 2 X 10(7) cells). The loss of MA from viable cells was monitored by membrane immunofluorescence and antibody-dependent cellular cytotoxicity. Soluble MA was demonstrated in papain digests through inhibition of antibody-dependent cellular cytotoxicity and by inhibition of the binding of anti-MA antibodies to cells as detected by use of 125I-labeled staphylococcal protein A. Approximately 75% of the MA activity in the extracts was not sedimentable at 100,000 X g,, indicating that the majority of EBV MA activity that was released by this procedure was associated with small-molecular-weight material. Antiserum prepared from an owl monkey immunized with these papain extracts contained antibody to MA and neutralizing antibodies, but lacked detectable antibodies against viral capsid antigens and EBV-induced early antigens.
MeSH terms
Animals; Antibodies, Viral; Antigens, Neoplasm; Antigens, Viral; Cell Line; Cell Membrane; Cytotoxicity Tests, Immunologic; Epitopes; Fluorescent Antibody Technique; Haplorhini; Herpesvirus 4, Human; Papain; Solubility
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