Analysis of the transfer region of the Streptomyces plasmid SCP2.
Mol Microbiol, 1993/10;10(1):157-70.
Brolle DF[1], Pape H, Hopwood DA, Kieser T
Affiliations
PMID: 7968512
Impact factor: 3.979
Abstract
pIJ903, a bifunctional derivative of the 31.4 kb low-copy-number, conjugative Streptomyces plasmid SCP2*, was mutagenized in Streptomyces lividans using Tn4560. Mutant plasmids differing in their transfer frequencies, chromosome mobilization abilities, pock formation, and complementation properties were isolated. The mutations defined five transfer-related genes, traA, traB, traC, traD and spd, clustered in a region of 9 kb. The deduced sequences of the putative TraA and TraB proteins showed no overall similarity to known protein sequences, but the phenotype of traA mutant plasmids and sequence motifs in the putative TraA protein suggested that it might be a DNA helicase.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; Base Sequence; Conjugation, Genetic; Escherichia coli Proteins; Fimbriae Proteins; Genes, Bacterial; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Insertional; Phenotype; Plasmids; Streptococcus
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