Toluidine blue staining of cartilage proteoglycan subunits.

Acta Histochem, 1978;61(1):127-34.

Geyer G, Linss W

PMID: 79294

Impact factor: 2.147

Abstract
Selective binding of toluidine blue to basophilic proteoglycans is the first stage of a staining method which proceeds to the formation of a heavy metal salt of the dyestuff. By means of this procedure matrix complexes of hyalin cartilage were stained such as to display granular subunits about 25 to 35 A in diameter. The findings are interpreted as the demonstration of proteoglycan constituents of chondromucin aggregates.
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