Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries.
J Virol, 1978/5;26(2):536-9.
PMID: 77913
Impact factor: 6.549
Abstract
Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.
MeSH terms
Cell Line; Clone Cells; Cytological Techniques; Moloney murine leukemia virus; RNA-Directed DNA Polymerase; Virus Cultivation
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