Polymeric cryoprotectants in the preservation of biological ultrastructure. III. Morphological aspects.
J Microsc, 1977/8;110(3):257-70.
Skaer HB, Franks F, Asquith MH, Echlin P
PMID: 73593
Impact factor: 1.952
Abstract
Two high molecular weight polymers, polyvinylpyrrolidone (PVP) and hydroxyethyl starch (HES), have been used as cryoprotectants for preparing specimens to be freeze fractured. Solutions of 25% (w/w) suppress the formation of intracellular ice in single cells and tissue blocks from both plants and animals to the extent that fine structural details of the cell can be elucidates. The mode of action of these cryoprotectants, together with the structures they reveal and the peculiar advantages attached to their use, is discussed.
MeSH terms
Cryoprotective Agents; Freeze Fracturing; Hydroxyethyl Starch Derivatives; Methods; Microscopy, Electron; Molecular Weight; Polymers; Povidone; Preservation, Biological
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