Micro-ELISA for detecting and assaying snake venom and venom-antibody.
Lancet, 1977/9/24;2(8039):639-41.
Theakston RD, Lloyd-Jones MJ, Reid HA
PMID: 71452
Impact factor: 202.731
Abstract
Enzyme-linked immunosorbent assay (ELISA) can detect venom levels down to 1-5 ng/ml. The assay is specific; no cross-reaction of clinical importance was found in a range of 14 different types of venom. Both venom and venom-antibody levels were monitored in mice. Specific antibody was detected in human serum more than 2 years after an accidental bite by Echis carinatus. ELISA should clarify many basic problems of envenoming in man.
MeSH terms
Animals; Antibodies; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Humans; Immune Sera; Immunoenzyme Techniques; Methods; Mice; Rabbits; Snake Venoms; Spectrophotometry
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