Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification.
Physiol Chem Phys, 1975;7(5):409-14.
PMID: 698
Abstract
The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.
MeSH terms
ATP Citrate (pro-S)-Lyase; Adenosine Monophosphate; Enzyme Activation; Hydrogen-Ion Concentration; Kinetics; Klebsiella; Oxo-Acid-Lyases
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