Comparison of DMSO and glycerol as cryoprotectants for ascites tumor cells.
Experientia, 1983/3/15;39(3):327-8.
PMID: 6825805
Abstract
Mouse Ehrlich ascites and rat D23 ascites tumors were stored in liquid nitrogen under identical conditions for up to 3 years. Cell viability (trypan blue exclusion) and transplantability of both tumors in animals remained virtually unaffected if preserved in 10% DMSO containing medium, whereas, cells preserved in 10% glycerol failed to produce lethal tumors in rodents.
MeSH terms
Animals; Ascites; Carcinoma, Ehrlich Tumor; Cell Survival; Dimethyl Sulfoxide; Freezing; Glycerol; Male; Mice; Neoplasm Transplantation; Neoplasms, Experimental; Rats; Time Factors; Tissue Preservation
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