H-2 restriction of virus-specific cytotoxicity across the H-2 barrier. Separate effector T-cell specificities are associated with self-H-2 and with the tolerated allogeneic H-2 in chimeras.
J Exp Med, 1976/10/01;144(4):933-45.
PMID: 62016
Impact factor: 17.579
Abstract
During infection with lymphocytic choriomeningitis or vaccinia virus, F1 irradiation chimeras reconstituted with bone marrow cells from or both parents generate cytotoxic T cells which can lyse targets across the H-2 barrier. However, activity of chimera T cells is H-2 restricted as shown by cold target competition experiments and selective restimulation of a secondary response in vitro; T cells of H-2k specificity which lyse tolerated infected H-2d target cells do not lyse infected H-2k or unrelated target cells and vice versa. Therefore, H-2 restriction of virus-specific cytotoxic T cells probably does not reflect need for like-like self-interactions for lysis to occur. The specificity of virus immune T cells is thus determined by the H-2K and H-2D specificities present in the infected animal and which are probably recognized unidirectionally by T cells. The results are compatible with the idea the T cells are specific for "altered alloantigen", i.e., a complex of cell surface marker and viral antigen. Alternatively, explained with a dual recognition model, T cells may possess two independently, clonally expressed receptors, a self-recognizer which is expressed for one of the syngeneic or tolerated allogeneic K or D "self" markers, and an immunologically specific receptor for viral antigen.
MeSH terms
Animals; Antigens, Viral; Cell Membrane; Crosses, Genetic; Cytopathogenic Effect, Viral; Cytotoxicity Tests, Immunologic; Epitopes; Female; Histocompatibility Antigens; Lymphocytic choriomeningitis virus; Male; Mice; Mice, Inbred Strains; Radiation Chimera; T-Lymphocytes; Transplantation, Homologous; Vaccinia virus
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