Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.
Nucleic Acids Res, 1976/6;3(6):1473-86.
PMID: 60740
Impact factor: 19.16
Abstract
The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.
MeSH terms
Avian Leukosis Virus; Avian Myeloblastosis Virus; DNA, Viral; Kinetics; Magnesium; Manganese; Polynucleotides; Potassium Chloride; RNA-Directed DNA Polymerase; Templates, Genetic
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