Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA.
Proc Natl Acad Sci U S A, 1976/5;73(5):1576-80.
PMID: 5725
Impact factor: 12.779
Abstract
Four site-specific endodeoxyribonucleases have been partially purified from extracts of bacteriophage T5-infected Escherichia coli by gel filtration and affinity chromatography on single- and double-stranded DNA. The enzymes were detected and characterized by agarose gel electrophoresis of alkali-denatured digestion products. None of the four is found in uninfected cells. In the presence of a divalent cation, all four endonucleases make ligase-repairable, single-chain interruptions at specific sites in the duplex DNA of several bacteriophages (lambda, T7, and T5) and a mammalian virus (adenovirus 2). These activities are not stimulated by ATP. None of the four is active on single-stranded DNA. The fragments produced by each enzyme from ligase-repaired T5 DNA do not correspond to those derived from mature T5 DNA. Each of the enzymes is able to cleave the intact strand of T5 DNA.
MeSH terms
Coliphages; DNA, Viral; Endonucleases; Hydrogen-Ion Concentration; Kinetics; Nucleic Acid Denaturation; Polynucleotide Ligases; Structure-Activity Relationship
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