Enzymic analysis of cardiac biopsy material from patients with valvular heart-disease.
Lancet, 1976/2/07;1(7954):269-70.
Peters TJ, Brooksby IA, Webb-Peploe MM, Wells G, Jenkins BS, Coltart DJ
PMID: 55585
Impact factor: 202.731
Abstract
Cardiac tissue obtained by left-ventricular endomyocardial biopsy from patients with valvular heart-disease was assayed for marker enzyme activities of subcellular organelles and these were correlated with left ventricular function as assessed by haemodynamic studies. In patients with poor left ventricular function, calcium-dependent adenosine-triphosphatase (A.T.P.ase) activity, predominantly localised to the myofibrils, was strikingly reduced. Activity of lactate dehydrongenase, a cytosol enzyme, was significantly increased in tissue from patients with poor left ventricular function. The activity of enzymes associated with sarcolemma (5'-nucleotidase), mitochondria (glutamate dehydrogenase and monoamine oxidase), microsomes (neutral alpha-glucosidase), and lysosomes (acid phosphatase, N-acetyl-beta-glucosaminidase) was no different in patients with good or poor left ventricular function. It is suggested that reduced myofibrillary A.T.P.ase concentration is the biochemical basis for the impaired ventricular function.
MeSH terms
Acid Phosphatase; Adenosine Triphosphatases; Biopsy; Glucosidases; Glutamate Dehydrogenase; Heart Valve Diseases; Heart Ventricles; Hemodynamics; Hexosaminidases; Humans; L-Lactate Dehydrogenase; Malate Dehydrogenase; Monoamine Oxidase; Myocardium; Nucleotidases
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