Reversed transport of amino acids in Ehrlich cells.
Biochim Biophys Acta, 1975/12/01;413(2):252-64.
PMID: 53074
Abstract
Gramicidin induces a marked Na+-dependent efflux of amino acids from Ehrlich cells. In absence of Na+, gramicidin does not alter the efflux. In presence gramicidin, glycine efflux is inhibited by methionine and less so by leucine. Glycine efflux caused by HgCl2 is neither Na+ dependent nor inhibitable by amino acids. Neither efflux of inositol which is transported by an Na+-dependent route, nor efflux of several other solutes which are transported by Na+-independent routes, is affected by gramicidin. The antibiotic appears to permit a reversal in the direction of of the operation of the Na+-dependent amino acid transport system. The increased efflux is partly, but not entirely, due to an increase in the cellular Na+ concentration and a reduction of the electrochemical potential difference for Na+.
MeSH terms
Amino Acids; Animals; Biological Transport; Carcinoma, Ehrlich Tumor; Cell Membrane; Glycine; Gramicidin; Inositol; Kinetics; Mice; Sodium; Temperature
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