Antigen-binding T cells as helper cells. Separation of helper cells by immune rosette formation.
J Exp Med, 1975/10/01;142(4):1035-9.
PMID: 51900
Impact factor: 17.579
Abstract
The spleen T cells from mice immunized 6 days earlier with either chicken gamma globulin (CGG) or with donkey erythrocytes (DRC) were rosetted with CGG-coated sheep erythrocytes or with DRC. The immune rosettes (RFC) (antigen-binding cells) were separated from the bulk of nonrosette-forming cells (non-RFC) by 1-g velocity sedimentation and the RFC and non-RFC tested for helper activity in cooperative antihapten responses in vitro. RFC or non-RFC were mixed with normal or hapten-primed spleen cells, challenged with the appropriate hapten-carrier conjugate and cultured for 4 days in Marbrook tissue cultures. The helping activity was quantitated from the numbers of antihapten antibody-producing cells generated per culture. The results show that specific helper cell activity could be selectively recovered in the immune rosette-forming cell population whereas the non-RFC population was depleted of help. These findings indicate that the helper T cells express specific antigen binding receptors.
MeSH terms
Animals; Antibody-Producing Cells; Antigens; Binding Sites, Antibody; Epitopes; Immune Adherence Reaction; Immunization; Mice; Spleen; T-Lymphocytes
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download