Calcium transport and translocation of adenine nucleotides in mitochondria from Morris hepatoma 3924A.
Cancer Res, 1979/7;39(7 Pt 1):2737-42.
Eboli ML, Malmström K, Galeotti T, López-Alarcón L, Carafoli E
PMID: 445477
Impact factor: 13.312
Abstract
The interaction of Ca2+ with Morris hepatoma 3924A mitochondria and its effect on the adenine nucleotide translocation have been studied. The characteristics of the Ca2+ transport process in mitochondria from Morris hepatoma are not significantly different from those of normal liver mitochondria. The Km for Ca2+ is 2 to 3 microM, and the rate versus concentration curve exhibits hyperbolic kinetics. A lower activity of the adenine nucleotide translocation was found, probably due to the high endogenous Ca2+ content of Morris hepatoma mitochondria (123 +/- 15 nmol Ca2+ per mg protein). No further inhibition of the translocase activity was observed after isolated mitochondria had accumulated extra amounts of Ca2+. The total amount of adenine nucleotides in tumor mitochondria is one-half those present in control liver, and a significantly lower percentage of the pool is present as adenosine 5'-monophosphate.
MeSH terms
Adenine Nucleotides; Animals; Biological Transport; Calcium; Kinetics; Liver Neoplasms, Experimental; Mitochondria; Mitochondria, Liver; Mitochondrial ADP, ATP Translocases; Nucleotidyltransferases; Rats; Rats, Inbred ACI
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