Preparation of specific immune sera against rabbit skeletal, pig and human cardiac heavy meromyosins.
Biomedicine, 1977/1/31;27(1):22-6.
Schwartz K, Sebag C, Bouveret P, Klotz C, Swynghedauw B
PMID: 403969
Abstract
Antibodies were produced in guinea-pigs against heavy-meromyosin (HMM) and tested by immunodiffusion and microcomplement fixation (MCF). A similar response was obtained for HMM extracted either from human and pig left ventricles, or from rabbit white skeletal muscles. For each muscle, the antisera cross-react quantitatively with the native intact myosin and are highly specific. Thus, this procedure permits the preparation of muscle type-specific anti myosin antibodies, by a simpler means than those previously published.
MeSH terms
Animals; Complement Fixation Tests; Guinea Pigs; Humans; Immune Sera; Immunization; Immunodiffusion; Muscles; Myocardium; Myosin Subfragments; Myosins; Rabbits; Species Specificity; Swine; Time Factors; Trypsin
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