The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation.
J Pharm Pharmacol, 1979/7;31(7):444-7.
PMID: 38312
Impact factor: 4.81
Abstract
A comparison was made of various indices of the hepatic drug-metabolizing apparatus associated with the postmitochondrial superntant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone-pretreated and 3-methylcholanthrene-pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P-450 and by the activity of NADPH-cytochrome c reductase, 4-dimethylaminoantipyrine N-demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes.
MeSH terms
Animals; Benzopyrene Hydroxylase; Centrifugation, Density Gradient; Cytochrome P-450 Enzyme System; Cytochromes; Enzyme Induction; Isoelectric Point; Liver; Male; Methylcholanthrene; Microsomes, Liver; NADPH-Ferrihemoprotein Reductase; Oxidoreductases, N-Demethylating; Phenobarbital; Rats
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