Preparation and some properties of human serum kallikrein immobilized on ARM-Sepharose.
Clin Chim Acta, 1979/5/02;93(3):329-33.
Kawalec M, Kawiak J, Yarovaya GA, Dotsenko VL, Orekhovich VN
PMID: 36246
Impact factor: 6.314
Abstract
A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. KM values for N-benzoyl-L-arginine ethyl ester and N-tosyl-L-arginine methyl ester hydrolysis of this preparation were 1.10 x 10(-3) M and 3.6 x 10(-4) M, respectively; pH optimum of hydrolysis of these esters were found to be 8.2 and 8.5, respectively. The immobilized kallikrein possessed kininogenase activity and was capable of activating prekallikrein.
MeSH terms
Arginine; Enzymes, Immobilized; Humans; Hydrogen-Ion Concentration; Kallikreins; Tosylarginine Methyl Ester
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