Molecular basis of base substitution hotspots in Escherichia coli.
Nature, 1978/8/24;274(5673):775-80.
Coulondre C, Miller JH, Farabaugh PJ, Gilbert W
PMID: 355893
Impact factor: 69.504
Abstract
In the lacI gene of Escherichia coli spontaneous base substituion hotspots occur at 5-methylcytosine residues. The hotspots disappear when the respective cytosines are not methylated. We suggest that the hotspots may result from the spontaneous deamination of 5-methylcytosine to thymine, which is not excised by the enzyme DNA-uracil glycosidase.
MeSH terms
2-Aminopurine; Base Sequence; Codon; Cytosine; Escherichia coli; Genes; Lactose; Mutagens; Mutation; Peptide Chain Termination, Translational; Ultraviolet Rays
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