New immunofluorescent antibody test for diagnosis of candidiasis using macrophages-engulfed antigens as substrate.
J Immunol Methods, 1978;20:357-63.
PMID: 349086
Impact factor: 2.287
Abstract
A new method for detection of antibodies to polysaccharidic antigens of Candida albicans is described. The method is based on the selective uptake of carbohydrate-rich antigens by mouse peritoneal macrophages, the antibodies being revealed by indirect immunofluorescence after incubation of fixed, antigen-containing macrophages with sera (MU/IFA test). This test shows greater sensitivity than counterimmunoelectrophoresis individuals. The combination of CIE using cytoplasmic antigens and MU/IFA using surface antigens and MU/IFA using surface antigens is useful in a considerable majority of cases for diagnosis of clinical infection.
MeSH terms
Animals; Antibodies, Anti-Idiotypic; Antigens, Fungal; Candidiasis; Counterimmunoelectrophoresis; Female; Fluorescent Antibody Technique; Humans; Immunoglobulin G; Macrophages; Mice; Mice, Inbred DBA; Rabbits
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