The lability of the products of mitochondrial protein synthesis in Saccharomyces cerevisiae. A novel method for protein half-life determination.
Biochem J, 1978/3/15;170(3):569-76.
Bakalkin GY, Kalnov SL, Galkin AV, Zubatov AS, Luzikov VN
PMID: 348203
Impact factor: 3.766
Abstract
A method for the determination of the half-life of mitochondrial translation products in yeast in vivo is proposed. The method uses inhibitors of cytoplasmic and mitochondrial protein synthesis and is based on double-labelling pulse-chase techniques, the second label being used to estimate 'post-incorporation' during the 'chase'. For the first time the difference between post-incroporation and the widely known recycling of the label is considered. These studies show that, in the turnover of mitochondrial translation products, the problem is of post-incorporation into mitochondria (especially from the cell sap) is predominant. The results obtained with this procedure indicate that the half-life of the products of mitochondrial protein synthesis in yeast at the late-exponential phase is about 60 min. The results suggest that mitochondrial transplantation products are subject to proteolysis to acid-soluble forms.
MeSH terms
Chloramphenicol; Cycloheximide; Fungal Proteins; Half-Life; Methods; Mitochondria; Oxygen Consumption; Saccharomyces cerevisiae
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