Initiation of lambda DNA replication in vitro promoted by isolated P-gene product.
Eur J Biochem, 1978/2/01;83(1):59-66.
Klein A, Bremer B, Kluding H, Symmons P
PMID: 342246
Abstract
The product of the P gene of bacteriophage lambda was isolated from heat-induced lambda-lysogenic Escherichia coli cells. It was found to bind to DNA, to be devoid of nuclease activity acting on double-stranded lambda DNA and of nicking/closing activity. Initiation of lambda DNA replication promoted by the P-gene product in a complementation assay in vitro was sensitive to rifampicin. Sedimentation analysis of the products and their hybridization to separated lambda DNA strands indicate that lambda DNA was formed in a reaction similar to ring-to-ring replication in vivo. The reaction was symmetric from the beginning, i.e. both lambda DNA strands were copied without delay.
MeSH terms
Coliphages; DNA Replication; DNA, Viral; Escherichia coli; Genes, Viral; Genetic Complementation Test; Kinetics; Lysogeny; Molecular Weight; Viral Proteins; Virus Replication
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