Electrophysiological evidence for the autoregulation of beta-cell secretion by insulin.
Biochim Biophys Acta, 1977/4/27;497(2):408-14.
Pace CS, Matschinsky FM, Lacy PE, Conant S
PMID: 322725
Abstract
Electrophysiological studies of cultured rat pancreatic beta-cells using intracellular microelectrodes show that exogenous insulin over the range of 0.1 -- 10.0 microng/ml inhibits the electrical activity due to 27.8 mM glucose in a dose-related manner. This inhibitory effect is manifested by a mean increase of the membrane potential from about --20 to --30 mV and inhibition of the number of cells impaled showing spike activity from 60 to less than 10%. The inhibitory influence of insulin is rapid occurring within 5 min for the highest level used. The results provide evidence for a negative feedback role of insulin in regulating its own release.
MeSH terms
Animals; Cells, Cultured; Electric Conductivity; Glucose; Insulin; Insulin Secretion; Islets of Langerhans; Male; Rats
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