Mapping of a gene for a major outer membrane protein of Escherichia coli K12 with the aid of a newly isolated bacteriophage.
Mol Gen Genet, 1977/1/07;150(1):103-5.
Verhoef C, de Graaff PJ, Lugtenberg EJ
PMID: 319339
Abstract
A method is described for the enrichment of phages which can adsorb to a specific determinant of bacterial cell surfaces. A phage was isolated which absorbs to E. coli cells containing the "major outer membrane= protein c but not to strains that are lacking this protein. With the aid of this phage a gene, meoA which is responsible for the lack of protein c was mapped at 48 min on the linkage map of E. coli K12.
MeSH terms
Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Coliphages; Escherichia coli; Genes; Membrane Proteins; Transduction, Genetic; Viral Plaque Assay
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