Physical mapping of Staphylococcus aureus penicillinase plasmid pI524: characterization of an invertible region.
Mol Gen Genet, 1979/8;175(1):19-30.
PMID: 316096
Abstract
The staphylococcal penicillinase plasmid pI524 and a series of derivatives have been extensively mapped by restriction endonuclease digestion and by heteroduplex analysis. We report here the identification of a 2.2 kb region that undergoes a reversible, rec-independent inversion. This sequence is bounded by a pair of inverted repeats 650 base pairs in length, and has asymmetrically located recognition sites for at least three restriction endonucleases. A series of deleted derivatives and one naturally occurring, closely related plasmid, were studied. Two of these retain the inversion; the remainder are incapable of inverting and were all found to be locked in the same orientation of the inversion. The invertible sequence is adjacent to the region of the plasmid encoding beta-lactamase (bla); this entire region appears to be transposable and the inversion may be involved in the regulation of beta-lactamase expression or in translocation.
MeSH terms
DNA Restriction Enzymes; DNA, Bacterial; Genes; Genetic Linkage; Penicillinase; Plasmids; Staphylococcus aureus; beta-Lactamases
More resources
EndNote: Download