[Production of lymphotoxin by human lymphocytes. II. Stimulation by purified tuberculin].
Zh Mikrobiol Epidemiol Immunobiol, 1979/9;(9):32-6.
Reĭzenbruk VG, Tapupere VO, Kremerman IB
PMID: 315144
Abstract
A total of 43 lymphocyte culture (LC) from 12 adults (aged 32--48 years) and 21 children 6--7 years of age were studied. In PPD-stimulated adults LC stimulation ratio ranged from 8 to 39%; their cytotoxic activity, i.e. lymphotoxin (LT) production, was determined by staining target L cells with crystal violet and measuring protein synthesis in surviving target cells by radioactive amino acid incorporation. In children blastogenic response occurred in 11 out of 21 PPD-stimulated LC studied, but only 8 supernatant culture fluids were toxic to L cells and 3 were not. These findings were confirmed by repeated tests 3 weeks later. Probable correlation of BTL and LT production with the functional state of specific immunity to tuberculosis is discussed.
MeSH terms
Adult; Cells, Cultured; Child; Humans; L Cells; Lymphocyte Activation; Lymphocytes; Lymphotoxin-alpha; Middle Aged; Phytohemagglutinins; Tuberculin; Tuberculosis
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