Separation of rat T and B lymphocytes by density gradient electrophoresis.
J Immunol Methods, 1979;26(3):245-51.
PMID: 312887
Impact factor: 2.287
Abstract
Density gradient electrophoresis has been employed for the preparative separation of T and B lymphocytes from rat spleen and peripheral blood. The high mobility cells were found to be predominantly T lymphocytes, as determined by their response to phytohemagglutinin and the relative absence of immunoglobulin-positive cells. The low mobility cells were predominantly B cells, as determined by the high percentage of immunoglobulin-positive cells and the total lack of response to PHA, an exclusive T cell mitogen. A better separation of peripheral blood T and B lymphocytes was achieved than with spleen T and B cells.
MeSH terms
Animals; B-Lymphocytes; Blood Cells; Cell Separation; Centrifugation, Density Gradient; Electrophoresis; Male; Rats; Spleen; T-Lymphocytes
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