Induction of partial chimerism in nonirradiated B-lymphocyte-deficient CBA/N mice.
J Exp Med, 1978/3/01;147(3):940-5.
Volf D, Sensenbrenner LL, Sharkis SJ, Elfenbein GJ, Scher I
PMID: 305463
Impact factor: 17.579
Abstract
Nonirradiated B-lymphocyte-deficient CBA/N mice given T6T6 chromosome-marked normal CBA/CaHN spleen cells became lymphoid chimeras exhibiting donor-type mitoses. Normal CBA/CaHN recipients did not exhibit significant numbers of donor-type mitoses. The lymphoid cell chimerism in the CBA/N host appeared in spleen, lymph nodes, and Peyer's patches, but not in marrow or thymus. Stimulation of CBA/N-recipient spleen cells in vitro suggested that the chimerism involved donor T6T6 cells which were responsive to the B-lymphocyte mitogen, lipopolysaccharide, but not to the T-lymphocyte mitogen, phytohemagglutinin. These data indicate that stable, long-term chimerism of a specific class of lymphocytes is possible in nonirradiated, B-lymphocyte-deficient CBA/N mice.
MeSH terms
Animals; B-Lymphocytes; Cells, Cultured; Chimera; Lymphocyte Activation; Lymphoid Tissue; Mice; Mice, Inbred CBA; Mitosis; Spleen; Transplantation, Homologous
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