Cell wall autolysis in log phase cells of Micrococcus lysodeikticus (luteus).
Microbiol Immunol, 1978;22(2):57-66.
Monodane T, Matsushima Y, Hirachi Y, Kotani S
PMID: 27703
Impact factor: 2.962
Abstract
Log phase cells of Micrococcus lysodeikticus (luteus) IFO 3333 autolyzed when incubated at 37 C in 0.01 M sodium-phosphate buffer pH 7.5. The enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n-butanol, and proved to be an N-acetylmuramyl-L-alanine amidase. The autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer.
MeSH terms
Agar; Alanine; Amidohydrolases; Autolysis; Buffers; Cell Wall; Culture Media; Cytoplasm; Deoxyribonucleases; Hydrogen-Ion Concentration; Micrococcus; Muramic Acids; Sucrose; Trypsin
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