[Stableness of the alkaline protease of the fungus Torula thermophila strain UzPT-1 to heating and the action of Ca2+ ions].
Prikl Biokhim Mikrobiol, 1975/9-1975/10;11(5):704-10.
PMID: 241993
Abstract
Thermal stability of protease of the fungus Torula thermophila strain UzPT-1 was examined. The dependence of this property on the content of monomer and dimer forms in the preparation was also investigated. The preparations with a high content of the dimer form showed greater thermal stability in distilled water. The preparations/with the predominant monomer from retained thermal stability in buffer solutions at pH 8 and lost it in distilled water when stored in the cold (2-4 degrees C) for several days. The preparations which lost thermal stability upon ammonium sulphate precipitation recovered partially or completely after addition of Ca2+ ions at a concentration of 0.001 M.
MeSH terms
Calcium; Cations, Divalent; Chromatography, Gel; Cryptococcus; Drug Stability; Hydrogen-Ion Concentration; Peptide Hydrolases; Solutions; Temperature
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